Development of Sex Specific Molecular Markers in Dioecious Piper Longum.l Plants by Differential Display

In the absence of any genetic or sequence information of a closely related species or individuals, showing morphological similarity and limited difference, differential display becomes an important technique to look into their genetic similarity and differences. Plants showing difference like disease resistance and susceptibility can be screened by this technique and sequence homology search can be carried out. Once some potent cDNA fragments were identified, they can be sequenced for full length gene and also design specific primers to screen the particular trait. Using this technique we could identify male and female plants of Piper longum at a younger stage.